High-Resolution Sampling 2D-LC Analysis of Mangosteen Pericarp Xanthones Using the Agilent 1290 Infinity II 2D-LC Solution

A high-resolution UHPLC analysis of xanthones present in the extracts of the pericarp of mangosteen (Garcinia mangostana L.) was carried out on an Agilent 1290 Infinity II LC. Excellent resolution and peak capacity (300 calculated at half height) were obtained within a total analysis time, including column reconditioning, of 16.5 minutes. The main xanthone (α-mangostin) was quantified in two extracts. To ensure and evaluate the purity of target compound peaks, a significant increase in peak capacity is required. Therefore, the analysis was repeated using high-resolution sampling 2D-LC with the Agilent 1290 Infinity II 2D-LC solution. The original separation was coupled online to a second-dimension separation for more resolution on a selection of peaks eluting from the first dimension. Diode array detection (DAD) and mass spectrometry (MS) were used for detection and